Coding

Part:BBa_K2022003:Design

Designed by: Gavin Sutton   Group: iGEM16_UNSW_Australia   (2016-10-11)


TolR, Periplasmic Domain, His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See: https://parts.igem.org/wiki/index.php?title=Part:BBa_K2022002

The his-tag was created by adding six histidine residues just prior to double stop codons that stop translation. The DNA sequence for these six His residues is catcaccatcaccatcac, which is (roughly) E. coli codon optimised by balancing between the two codons for His


Source

See: https://parts.igem.org/wiki/index.php?title=Part:BBa_K2022002

References